DNA Oligonucleotide Synthesis
Standard PCR Protocol
Oligos | Sigma-Aldrich
Oligos | Sigma-Aldrich
Taq DNA Polymerase with 10× PCR reaction buffer containing MgCl2 9012-90-2
Oligos | Sigma-Aldrich
Oligos, qPCR Probes & Peptides
Oligo(dT)23, Anchored 70 μM in H2O | Sigma-Aldrich
Polymerase Chain Reaction
SygRNA™ Cas9 Synthetic Modified tracrRNA | Sigma-Aldrich
Deoxynucleotide Set, 10 mM Individual dNTPs for routine PCR; 0.5 mL each | Sigma-Aldrich
5′/3′ RACE Kit, 2nd Generation sufficient for 10 reactions | Sigma-Aldrich
Custom DNA and RNA Oligos & qPCR Probes Product Resources
Oligonucleotide Quantification
Oligos | Sigma-Aldrich
PCR Core kit with Taq DNA polymerase PCR components in separate tubes to allow for optimization | Sigma-Aldrich
Custom iScale Oligos™
PCR/qPCR/dPCR Assay Design
Oligonucleotides | Sigma-Aldrich
Phosphorothioate Oligonucleotides
Oligonucleotide Melting Temperature
Oligonucleotides | Sigma-Aldrich
Antisense Oligonucleotides
Oligos | Sigma-Aldrich
Oligonucleotide Quantification
